2BJB
Mycobacterium Tuberculosis Epsp Synthase In Unliganded State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.864, 102.864, 99.512 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6t |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.888 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.810 |
| Number of reflections | 36380 | |
| <I/σ(I)> | 17.5 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 1.6M NA ACETATE, pH 6.10 |
