2BJ0
Crystal Structure of AChBP from Bulinus truncatus revals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-19 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.945, 219.018, 166.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.523 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.770 |
| Number of reflections | 93560 | |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 98.0 | 89 |
| Redundancy | 5.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 0.1 M CAPS PH10.5, 0.2 M LITHIUMSULFATE, 2 M AMMONIUM SULFATE, pH 10.50 |
