2BIX
Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, Fe-free apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 122.928, 122.928, 205.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.680 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.850 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 44859 | |
| <I/σ(I)> | 24.5 | 7.2 |
| Completeness [%] | 98.6 | 92.6 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | pH 5.70 |
