2BIR

ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-11-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	49.610, 48.490, 40.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.216
Rwork	0.216
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bir
RMSD bond length	0.032
RMSD bond angle	26.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.107 *	0.239 *
Number of reflections	4313
<I/σ(I)>	5.5	3.1
Completeness [%]	93.0 *	49.7 *
Redundancy	5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.2		pH 4.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	reservoir	2'-GMP	0.125 (%(w/v))
4	1	reservoir		1.25 (%(w/v))
5	1	reservoir	MPD	50 (%(v/v))