2BIQ
human p53 core domain mutant T123A-M133L-H168R-V203A-N239Y-R249S- N268D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 45.480, 45.480, 331.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.400 - 1.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uol |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 20201 | |
| <I/σ(I)> | 22.1 | 4.3 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 9.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
