2BIL
The human protein kinase Pim1 in complex with its consensus peptide Pimtide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-04 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 65 |
| Unit cell lengths | 98.100, 98.100, 80.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.500 - 2.550 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xws |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.974 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.480 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.130 | 0.510 |
| Number of reflections | 14259 | |
| <I/σ(I)> | 9.7 | 2.7 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 6.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M MSPG, 30% PEG1K, 0.5% DMSO, SITTING DROP, VAPOR DIFFUSION, 277 K |
