2BII
crystal structure of nitrate-reducing fragment of assimilatory nitrate reductase from Pichia angusta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.722, 123.080, 149.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.710 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bih |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 2235039 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
