2BHT

Crystal structure of O-acetylserine sulfhydrylase B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 41
Unit cell lengths	149.940, 149.940, 194.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.225
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bhs
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.100
Rmerge	0.060
Number of reflections	123872
<I/σ(I)>	14.9
Completeness [%]	99.8
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6		pH 7.60