2BGM
X-Ray structure of ternary-Secoisolariciresinol Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU STURN 92 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU CCD |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 57.350, 118.740, 131.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bgk |
| RMSD bond length | 0.020 |
| RMSD bond angle | 4.100 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 12187 | |
| <I/σ(I)> | 15.6 | 2.2 |
| Completeness [%] | 79.1 | 13.1 |
| Redundancy | 5.81 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
