2BGG
The structure of a Piwi protein from Archaeoglobus fulgidus complexed with a 16nt siRNA duplex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 51.916, 61.206, 104.059 |
Unit cell angles | 76.54, 76.14, 79.35 |
Refinement procedure
Resolution | 99.010 - 2.200 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w9h |
RMSD bond length | 0.022 |
RMSD bond angle | 2.097 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.400 |
Number of reflections | 58841 | |
<I/σ(I)> | 7.5 | 1.2 |
Completeness [%] | 96.7 | 96.5 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |