2BF8

Crystal structure of SUMO modified ubiquitin conjugating enzyme E2- 25K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Collection date	2003-09-07
Detector	ADSC ADSC Q4R
Spacegroup name	P 43 21 2
Unit cell lengths	58.391, 58.391, 162.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.050 - 2.300
R-factor	0.213
Rwork	0.210
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fzy
RMSD bond length	0.018
RMSD bond angle	1.640
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.530
Number of reflections	13257
<I/σ(I)>	30.6	3.8
Completeness [%]	99.9	99.5
Redundancy	13.4	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		100 MM TRIS PH 8.5 200 MM MAGNESIUM CHLORIDE 17% PEG 4000 10 % GLYCEROL