2BES
Structure of Mycobacterium tuberculosis Ribose-5-Phosphate Isomerase, RpiB, Rv2465c, in complex with 4-phospho-D-erythronohydroxamic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.479, 102.855, 69.545 |
| Unit cell angles | 90.00, 95.37, 90.00 |
Refinement procedure
| Resolution | 81.650 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 55757 | |
| <I/σ(I)> | 5.3 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.55 M AMMONIUM PHOSPHATE, 0.1 M HEPES, PH 7.5 |
