2BCZ
Crystal Structure of a minimal, mutant all-RNA hairpin ribozyme (U39C, G8I, 2'deoxy A-1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 83 |
Detector technology | CCD |
Collection date | 2005-05-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9764 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.490, 94.490, 127.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.300 - 2.400 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.27000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ZFR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.300 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.043 | 0.430 |
Number of reflections | 13689 | |
<I/σ(I)> | 19.1 | 4.3 |
Completeness [%] | 99.2 | 98.7 |
Redundancy | 6.25 | 5.91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG550 MME, lithium sulfate, spermidine, cobalt hexamine, , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG550 MME | ||
2 | 1 | 1 | lithium sulfate | ||
3 | 1 | 1 | spermidine | ||
4 | 1 | 1 | cobalt hexamine | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG550 MME | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | cobalt hexamine |