2BCX
Crystal structure of calmodulin in complex with a ryanodine receptor peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-03 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.100, 44.100, 90.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.990 - 2.000 |
R-factor | 0.214 |
Rwork | 0.213 |
R-free | 0.24900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data scaling software | d*TREK (9.1SSI) |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.660 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.038 | 0.129 |
Number of reflections | 10802 | |
<I/σ(I)> | 23.2 | 7.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 23% PEG 4000, 0.1 M sodium acetate, 5 mM DTT, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |