2BCT
THE ARMADILLO REPEAT REGION FROM MURINE BETA-CATENIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.090, 75.590, 134.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bct |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.257 |
| Number of reflections | 11818 | |
| <I/σ(I)> | 22.7 | 6.7 |
| Completeness [%] | 96.7 | 93.4 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 10.5 * | 10% (W/V) PEG 8000, 875 MM NACL, 200 MM TRIS-HCL, PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-59 | 7 (mg/ml) | |
| 2 | 1 | drop | CAPS | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | DTT | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 7 | 1 | reservoir | 875 (mM) |
