2BBO
Human NBD1 with Phe508
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Collection date | 2004-10-28 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.836, 57.836, 141.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.800 - 2.550 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xmi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.162 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 53.500 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 8125 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 280 | 30% PEG4000, 0.2M LiCl, 50mM Tris, pH 9, 3mM ATP, 5mM MgCl2, 2mM TCEP, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
