2BB7
Mn Form Of E. coli Methionine Aminopeptidase In Complex With a quinolinyl sulfonamide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.039, 60.416, 50.521 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 17.620 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.860 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 | 0.158 |
| Number of reflections | 24139 | |
| <I/σ(I)> | 11.5 | 4.6 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
