2BB5
Structure of Human Transcobalamin in complex with Cobalamin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-14 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.127, 145.497, 165.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.258 |
Rwork | 0.257 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF BOVINE TRANSCOBALAMIN IN MONOCLINIC CRYSTAL FORM (PDB 2BB6). |
RMSD bond length | 0.008 |
RMSD bond angle | 1.094 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.240 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.142 | 0.388 |
Number of reflections | 19417 | |
<I/σ(I)> | 4.5 | 1.9 |
Completeness [%] | 96.5 | 95.5 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 293 | 20% PEG 4000, 18% ethanol, 0.2M TRIS, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.60 |