2BB3
Crystal Structure of Cobalamin Biosynthesis Precorrin-6Y Methylase (cbiE) from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.191, 88.418, 115.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.270 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.808 |
| Number of reflections | 22771 | |
| <I/σ(I)> | 13.5 | 2.56 |
| Completeness [%] | 97.3 | 82.2 |
| Redundancy | 11 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M Sodium Acetate, 11% PEG10K, 2% Iso-Propanol,10mM SAH, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
