2BB0
Structure of Imidazolonepropionase from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.730, 106.340, 66.470 |
Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.22100 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.098 | 0.256 |
Number of reflections | 54728 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG4000, 0.1M Tris-HCl, 0.2M Sodium Acetate, 2% benzamidine hydrochloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |