2BAY

Crystal structure of the Prp19 U-box dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-01
Detector	MARRESEARCH
Wavelength(s)	1.381
Spacegroup name	P 21 21 21
Unit cell lengths	49.421, 57.105, 122.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.940 - 1.500
R-factor	0.18363
Rwork	0.182
R-free	0.20396
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.468
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.940	1.570
High resolution limit [Å]	1.500	1.500
Number of reflections	52748
Completeness [%]	93.3	86.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	15 mgs/ml and 33% Poly-Ethylene Glycol (PEG) 4000, 75 mM MgCl2, 0.1 M Tris pH 8.5, 1 mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	15 mgs/ml and 33% Poly-Ethylene Glycol (PEG) 4000, 75 mM MgCl2, 0.1 M Tris pH 8.5, 1 mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 277K