2BA1

Archaeal exosome core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.01
Spacegroup name	P 43 2 2
Unit cell lengths	137.460, 137.460, 261.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.224
R-free	0.27500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	69124
Completeness [%]	99.5	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1M Sodium acetate, 32% MPD, 10mM NaCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1M Sodium acetate, 32% MPD, 10mM NaCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K