2B9B
Structure of the Parainfluenza Virus 5 F Protein in its Metastable, Pre-fusion Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-31 |
Detector | MAR CCD 165 mm |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 160.206, 259.864, 154.012 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.150 - 2.850 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.25900 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.150 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.073 | 0.376 |
Number of reflections | 74802 | |
<I/σ(I)> | 7.1 | 2 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 295 | sodium potassium tartrate, imidazole, pH 8.00, temperature 295K |