2B97

Ultra-high resolution structure of hydrophobin HFBII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-24
Detector	MARRESEARCH
Wavelength(s)	0.7747
Spacegroup name	C 1 2 1
Unit cell lengths	78.544, 46.254, 34.687
Unit cell angles	90.00, 111.64, 90.00

Refinement procedure

Resolution	20.000 - 0.750
Rwork	0.130
R-free	0.14820
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1r2m
RMSD bond length	0.027
RMSD bond angle	0.066
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	0.760
High resolution limit [Å]	0.750	0.750
Number of reflections	132282
<I/σ(I)>	18.01	1.91
Completeness [%]	98.7	91.3
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.39	298	0.2 M lithium sulfate, 15% PEG2000, 0.1 M sodium HEPES pH 7.5, manganese dichloride 50mM , pH 7.39, VAPOR DIFFUSION, HANGING DROP, temperature 298K