2B8H
A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.516, 107.516, 338.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.14141 |
| Rwork | 0.139 |
| R-free | 0.18869 |
| Structure solution method | MIR |
| Starting model (for MR) | 7nn9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.366 |
| Number of reflections | 680297 | |
| <I/σ(I)> | 16.3 | 4.6 |
| Completeness [%] | 99.7 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 10mM HEPES, 50mM ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
