2B78

A putative sam-dependent methyltransferase from Streptococcus mutans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-17
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	168.472, 50.659, 53.963
Unit cell angles	90.00, 104.22, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.241
R-free	0.25900
Structure solution method	SIRAS
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	52534
<I/σ(I)>	11	2.77
Completeness [%]	94.5	74.1
Redundancy	4.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	PEG8000, Sodium Cacodylate,Magnesium Acetates, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	PEG8000, Sodium Cacodylate,Magnesium Acetates, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K