2B5M

Crystal Structure of DDB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	173
Detector technology	CCD
Collection date	2003-11-12
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.380, 133.834, 184.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 2.920
R-factor	0.23472
Rwork	0.232
R-free	0.27725
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.700
High resolution limit [Å]	2.920
Number of reflections	33473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 4000, sodium chloride, MES, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 4000, sodium chloride, MES, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K