2B4V
Structural Basis for UTP Specificity of RNA Editing TUTases From Trypanosoma Brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95667 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.573, 91.573, 163.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond angle | 0.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.800 |
| Rmerge | 0.065 | 0.662 |
| Number of reflections | 65185 | |
| <I/σ(I)> | 39.6 | 3 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 13.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 3.0 M KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 3.0 M KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
