2B4V
Structural Basis for UTP Specificity of RNA Editing TUTases From Trypanosoma Brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95667 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.573, 91.573, 163.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.22700 |
Structure solution method | SAD |
RMSD bond angle | 0.011 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.770 | 1.800 |
Rmerge | 0.065 | 0.662 |
Number of reflections | 65185 | |
<I/σ(I)> | 39.6 | 3 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 13.6 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 3.0 M KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 3.0 M KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |