2B4C
Crystal structure of HIV-1 JR-FL gp120 core protein containing the third variable region (V3) complexed with CD4 and the X5 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 225.993, 225.993, 97.968 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.319 |
| Rwork | 0.319 |
| R-free | 0.34900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RHH AND 1RZK |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.082 | |
| Number of reflections | 19372 | |
| <I/σ(I)> | 26.2 | 1.3 |
| Completeness [%] | 86.6 | 20.9 |
| Redundancy | 9.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.50 |
