2B3T
Structure of complex between E. coli translation termination factor RF1 and the PrmC methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.540, 77.470, 89.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| Rwork | 0.244 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.080 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.104 | 0.474 |
| Number of reflections | 14670 | 1229 |
| <I/σ(I)> | 12.25 | 3.82 |
| Completeness [%] | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 291 | ammonium sulfate, NaCl, glycerol, Na Hepes, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
