2B3P

Crystal structure of a superfolder green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.07
Spacegroup name	P 31 2 1
Unit cell lengths	88.456, 88.456, 69.346
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.990 - 1.400
R-factor	0.206
Rwork	0.206
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9c
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	21.300
High resolution limit [Å]	1.400
Number of reflections	60830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	Hepes-NaOH, sodium acetate, cadmium sulfate, pH 7.5, VAPOR DIFFUSION, temperature 298K