2B3B
Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.643, 134.874, 159.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.086 | 0.455 |
| Number of reflections | 181610 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 95.6 | 82.4 |
| Redundancy | 5.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 10% (w/v) Isopropanol, 0.1M Sodium Citrate pH5.6, 10% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
