2B25
Human putative tRNA(1-methyladenosine)methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.533, 157.106, 113.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.567 - 2.500 |
| Rwork | 0.247 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9g |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.366 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.570 | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.082 | 0.064 | |
| Number of reflections | 30305 | ||
| Completeness [%] | 99.6 | ||
| Redundancy | 5.7 | 5.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 11 % PEG3350, 0.2M lithium citrate, 0.1M BisTris, 10% PEG400, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
