2B1J
Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-24 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.040, 62.160, 54.223 |
| Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
| Resolution | 40.700 - 2.400 |
| R-factor | 0.187 |
| Rwork | 0.181 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3chy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.700 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 10174 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 97.3 | 96 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 6000, magnesium chloride, MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
