2B1E
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-02-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792, 0.9794, 0.9611 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.846, 60.605, 223.131 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.25000 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39711 | |
Completeness [%] | 99.4 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 3000, lithium sulfate, sodium cloride, dithiothreitol, L-cysteine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |