2B0T
Structure of Monomeric NADP Isocitrate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.046, 52.692, 124.035 |
| Unit cell angles | 90.00, 108.98, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.750 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 78653 | |
| <I/σ(I)> | 23.2 | 1.66 |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | polyethylene glycol 2000 MME, Tris-HCl, MgCl2., pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
