2B05
Crystal Structure of 14-3-3 gamma in complex with a phosphoserine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99190 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.571, 121.571, 313.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.200 - 2.550 |
| R-factor | 0.25933 |
| Rwork | 0.258 |
| R-free | 0.30175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bq0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.079 | 0.931 |
| Number of reflections | 77602 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG 3350, magnesium chloride, Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
