2AZT
Crystal structure of H176N mutant of human Glycine N-Methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.116, 83.279, 115.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.000 - 2.700 |
| R-factor | 0.23918 |
| Rwork | 0.236 |
| R-free | 0.28172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r74 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 21138 | |
| <I/σ(I)> | 28.6 | 4.5 |
| Completeness [%] | 96.2 | 93.5 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 289 | 10% PEG4000, 0.1M Na citrate, 5% glycerol, pH 5.8, VAPOR DIFFUSION, temperature 289K |
