2AZM
Crystal structure of the MDC1 brct repeat in complex with the histone tail of gamma-H2AX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.437, 75.609, 114.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.410 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.24850 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.123 | 0.420 |
| Number of reflections | 23006 | |
| <I/σ(I)> | 17.4 | 4.1 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | PEG 4000, NaCl, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP |
