2AZL
Crystal structure for the mutant F117E of Thermotoga maritima octaprenyl pyrophosphate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 151.514, 151.514, 64.606 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.230 - 2.800 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.27600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1v4e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 9443 | |
<I/σ(I)> | 27.5 | 4.2 |
Completeness [%] | 99.0 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na+HEPES, Li2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |