2AZC
HIV-1 Protease NL4-3 6X mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-19 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 100.242, 100.242, 97.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.880 - 2.010 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2az8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.830 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.880 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.050 | 0.378 |
| Number of reflections | 16751 | |
| <I/σ(I)> | 14.4 | 3.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.84 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 297.16 | ammonium sulfate, sodium acetate, sodium thiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 297.16K, pH 5.20 |
