2AYQ
3-ISOPROPYLMALATE DEHYDROGENASE FROM THE MODERATE FACULTATIVE THERMOPHILE, BACILLUS COAGULANS
Replaces: 1AYQExperimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.400, 114.400, 194.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ipd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.540 * |
| Data reduction software | WEIS |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.054 | |
| Total number of observations | 101970 * | |
| Number of reflections | 25540 | |
| Completeness [%] | 84.5 | 62.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 2 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 3 | 1 | precipitant | Tris-HCl | or PEG |
