2AYE
Crystal structure of the unliganded E2 DNA Binding Domain from HPV6a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.284 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.766, 106.886, 74.945 |
| Unit cell angles | 90.00, 121.68, 90.00 |
Refinement procedure
| Resolution | 65.650 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.185 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Code 1R8H |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.951 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.650 | 2.590 |
| High resolution limit [Å] | 2.300 | 2.500 |
| Rmerge | 0.051 | 0.149 |
| Number of reflections | 26868 | |
| <I/σ(I)> | 37.3 | 5.5 |
| Completeness [%] | 99.6 | 82.7 |
| Redundancy | 8.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | ammonium sulphate, Tris, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
