2AXQ
Apo histidine-tagged saccharopine dehydrogenase (L-Glu forming) from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-08-18 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.279, 85.279, 141.977 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.850 - 1.700 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e5l used monomer backbone only |
RMSD bond length | 0.014 |
RMSD bond angle | 1.407 |
Data reduction software | CrystalClear (D*TREK (MSC/RIGAKU)) |
Data scaling software | d*TREK (9.2D) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.850 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.056 | 0.368 |
Number of reflections | 64775 | |
<I/σ(I)> | 15.8 | 4.3 |
Completeness [%] | 97.6 | 95.9 |
Redundancy | 7.18 | 4.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 1.2M Ammonium sulfate, 25mM Bis-Tris, 6.5-7mg/mL enzyme, 50mM Tris-HCl pH 8.0, 150mM KCl, 75mM imidazole, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |