2AXP
X-Ray Crystal Structure of Protein BSU20280 from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR256.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97575 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.847, 99.944, 78.344 |
| Unit cell angles | 90.00, 125.29, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.500 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.333 |
| Number of reflections | 38099 | |
| <I/σ(I)> | 15 | 3.2 |
| Completeness [%] | 99.2 | 95.8 |
| Redundancy | 4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate (pH 6.5), 17% PEG 8k, 240 mM magnesium acetate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
