2AXN

Crystal structure of the human inducible form 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-01
Detector	MARRESEARCH
Wavelength(s)	1.3808
Spacegroup name	P 65 2 2
Unit cell lengths	102.580, 102.580, 259.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.209
Rwork	0.209
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1K6M
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.225
High resolution limit [Å]	2.100	2.100
Rmerge	0.107	0.686
Number of reflections	44651
<I/σ(I)>	16.9	1.5
Completeness [%]	92.8	87.6
Redundancy	9.6	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 4000, ethylene glycol, Tris, phoshate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K