2AWY

met-DcrH-Hr

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.7403
Spacegroup name	P 1
Unit cell lengths	33.160, 44.846, 47.538
Unit cell angles	92.76, 103.49, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.221
R-free	0.28000
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG4000, CaCl2, isopropanol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG4000, CaCl2, isopropanol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG4000, CaCl2, isopropanol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K