2AW9
Superoxide dismutase with manganese from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5499 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.833, 83.499, 65.495 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 62.990 - 2.700 |
| R-factor | 0.1701 |
| Rwork | 0.168 |
| R-free | 0.21418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y67 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.742 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11594 | |
| <I/σ(I)> | 20.3297 | |
| Completeness [%] | 92.6 | 71.7 |
| Redundancy | 5.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | magnesium chloride, tris-hydrochloride, PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
