2AVU

Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97964
Spacegroup name	P 61
Unit cell lengths	151.137, 151.137, 114.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.790 - 3.000
R-factor	0.217
Rwork	0.217
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.037	0.314
Number of reflections	29451
<I/σ(I)>	28.3	4.2
Completeness [%]	99.4	99.5
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium chloride, sodium acetate, ethylene imine polymer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium chloride, sodium acetate, ethylene imine polymer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K