2AUH

Crystal structure of the Grb14 BPS region in complex with the insulin receptor tyrosine kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2005-04-07
Spacegroup name	P 62
Unit cell lengths	127.182, 127.182, 65.925
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.270 - 3.200
R-factor	0.223
Rwork	0.223
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ir3
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.270	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.080	0.377
Number of reflections	9903
<I/σ(I)>	14.3
Completeness [%]	99.8	100
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1